In vitro hydroxyapatite formation of a tetracalcium phosphate and anhydrous dicalcium phosphate based dentine desensitiser: TRIS buffer vs artificial saliva

Background: Calcium phosphates (CPs) form hydroxyapatite (HA) in physiological solutions. These are commonly used to treat dentine hypersensitivity (DH) as they mimic the mineral composition of natural tooth. Aim: The present study aims characterise apatite formation ability a commercially available calcium phosphate TeethmateTM (TM) physiological-like media. Materials and Methods: In this study, 4mm (D) x 6mm (L) cylindrical samples TM were produced immersed tris(hydroxymethyl)aminomethane (TRIS) buffer (pH: 7.3) artificial saliva (AS) 6.5) for up 24 hours. This was followed by characterisation after immersion using 31P magic angle - nuclear magnetic resonance spectroscopy (MAS-NMR), X-ray powder diffraction (XRD) treated with material scanning electron microscopy (SEM). Results: MAS-NMR XRD analyses revealed that TRIS solution formed within approximately 6 hours immersion. change observed at around 12 soaked AS.  pH media increased increasing time. SEM analysis showed transitional phase structures exhibiting plate-like morphology. Conclusion: shows converts HA vitro rapidly provides an effective option treatment hypersensitivity.